An extract of the medicinal mushroom Agaricus blazei Murill can protect against allergy
© Ellertsen and Hetland; licensee BioMed Central Ltd. 2009
Received: 05 February 2009
Accepted: 05 May 2009
Published: 05 May 2009
Agaricus blazei Murill (AbM) is an edible Brazilian mushroom that has been used in traditional medicine for a range of diseases. It has been shown to have anti-infection and anti-tumor properties in the mouse, which are due to induction of Th1 responses. On the other hand, IgE-mediated allergy is induced by a Th2 response.
Since according to the Th1/Th2 paradigm an increased Th1 response may promote a reduced Th2 response, the aim was to examine whether AbM had anti-allergy effects.
A mouse model for allergy was employed, in which the mice were immunized s.c. with the model allergen ovalbumin (OVA). Additionally, the animals were given a mushroom extract, AndoSan™, mainly (82%) containing AbM, but also Hericium erinaceum (15%) and Grifola frondosa (3%), or PBS p.o. either a day before or 19 days after the immunization. The mice were sacrificed on day 26, and anti-OVA IgE (Th2 response) and IgG2a (Th1 response) antibodies were examined in serum and Th1, Th2 and Treg cytokines in spleen cells cultures.
It was found that the AndoSan™ extract both when given either before or after OVA immunization reduced the levels of anti-OVA IgE, but not IgG2a, in the mice. There was a tendency to reduced Th2 relative to Th1 cytokine levels in the AndoSan™ groups.
This particular AbM extract may both prevent allergy development and be used as a therapeutical substance against established allergy.
Agaricus blazei Murill (AbM) of the family Basidiomycetes is a popular edible medicinal mushroom, originally native to a small village, Piedade, in the highland areas of Atlantic forest near São Paulo, Brazil. It has traditionally been used for the prevention of a range of diseases, including cancer, hepatitis, atherosclerosis, hypercholesterolemia, diabetes and dermatitis [1, 2]. Because of its alleged health effects, the mushroom was brought to Japan in the mid-60-ies and subjected to biomedical research. AbM was found to be rich in immuno-modulating substances such as β-glucans [3, 4] and proteoglycans , and it had anti-infection [6, 7] and anti-tumor [4, 5] effects in mice.
Anti-tumor and anti-infection immunity are both due to Th1 responses, which also do promote autoimmune disease when overshooting. On the other hand, anti-helminth and anti-rejection immunity are due to Th2 responses, which may also induce IgE-mediated allergy, whereas delayed-type hypersensitivity is believed to involve Th1 cells. Since, according to the original Th1/Th2 dichotomy  there is an inverse relationship between Th1 and Th2 responses, we set out to look for substances that increased Th1 responses and thus, presumably, would reduce allergy. Moreover, we looked for substances with broad immunogenic specificity and hence a broad range of possible therapeutical activity. This criterion fits substances containing so-called pathogen-associated molecular patterns, which stimulate innate immunity via binding to a few different receptors with broad specificities like Toll-like receptors and dectin-1.
In order to test putative functional Th1-stimulating substances, a mouse model for systemic bacterial infection was chosen rather than a tumor model, because of the more rapid outcome of an anti-bacterial than an anti-tumor response. We tested different β-glucans, which are known stimulators of innate immunity with anti-tumor  and anti-infection  activities. We found that one β-1,3-glucan from Sclerotinia sclerotiorum was highly protective against sepsis in a mouse model for systemic S. pneumoniae, although only when given i.p. and not p.o. . However, surprisingly, we detected that s.c. administration of both this β-glucan and other β-glucans from barley and baker's yeast, in addition to moulds per se, also increased specific IgE levels in a mouse model for allergy [12, 13]. This is in agreement with the finding of increased allergic responses of mold-derived β-1,3-glucan in an airway inhalation model in the mouse . Since AbM is another more recently discovered source of strong innate stimulatory properties [15, 16], with a high content of β-glucan and anti-tumor properties in the mouse , we tested whether extracts of AbM from different producers had anti-infection effects in the said mouse model for pneumococcal sepsis. We found that the current extract, AndoSan™, containing approximately 80% of AbM and 20% of two other Basidiomycetes mushrooms; Hericium erinaceum and Grifola frondosa, was the most effective: It was the only extract that decreased bacteremia statistically significantly and increased the survival rate of the exposed animals . Moreover, it had more profound anti-infection effect even when given p.o. via a gastric catheter than did any of the above β-glucans given i.p..
There are anecdotes about persons who have used AbM for other purposes than allergy, and who have experienced less allergic symptoms when ingesting the remedy. To our knowledge the very few papers on AbM or other Basidiomycetes mushrooms and allergy in English scientific literature rather report on induction of allergy; cheilitis and increased delayed-type sensitivity due to AbM [18, 19], hypersensitivity pneumonitis caused by Grifola frondosa , and allergic contact dermatitis from Hericeum erinaceum exposure . Based on preliminary anti-infection and anti-allergy results in our laboratory with the current extract of mainly AbM (AndoSan™), a patent application was filed in 2004 . There are other publications on beneficial effects of the mushrooms in the patent literature, foremost of Japanese origin: One patent (A61K 35/84, 05.08.2002) claims that an essence extracted from mycelium of Basidiomycetes, including Hericium erinaceum, can prevent and cure allergic symptoms, especially atopic dermatitis. Another (WO 02/15917) claims the use of AbM in treatment of autoimmune and skin diseases, due to down-regulation of immune function. Yet another (WO 93/207923) describes the isolation from Agaricus hortensis of anti-allergic components, especially for dermatological usage. Extracts of AbM have also been found to have anti-allergic effect based on inhibition of basophilic leukocytes (US2003/0104006). Finally, EP0413053 describes a process for producing an anti-allergic substance from Basidiomycetes mycelium, including that of AbM and Grifola frondosa.
The aim of the present study was to examine whether the extract that was most effective against systemic pneumococcal infection, also could protect against allergy development when given to a mouse model for allergy. For this purpose the model allergen ovalbumin (OVA) was injected s.c. and AbM extract as adjuvant was given orally, and levels of specific IgE and IgG2a antibodies were determined in serum. In addition, Th1, Th2 and Treg cytokines were measured in supernatants of cultured spleen cells from the mice.
These were inbred, female, pathogen-free, 6–8 weeks old NIH/OlaHsd, C57Bl/6 and Balb/c obtained from Gl. Bomholt gård Ltd (Ry, Denmark) and rested for 1 week after arrival. They were housed 8 animals per cage, individually earmarked, and given water and egg-free feed ad libitum. Experiments were performed according to law and regulations for animal experiments in Norway, which are in agreement with the Helsinki declaration, and they were approved by the local Animal Board under the minister of Agriculture in Norway.
An aqueous extract of mycelium of AbM (82%), containing additionally Hericium erinaceum (15%) and Grifola frondosa (3%) (AndoSan™), grown commercially, was given by ACE Co., Ltd., Gifu, Japan. It was stored at 4°C in dark bottles and kept sterile until being instilled intragastrically in the mice. The AbM mixed powder contains per 100 g the following constituents: moisture 5.8 g, protein 2.6 g, fat 0.3 g, carbohydrates 89.4 g of which β-glucan constitutes 2.8 g, and ash 1.9 g, and its final concentration was 340 g/l. The amount per liter of the extract for sodium was 11 mg, phosphorus 254 mg, calcium 35 mg, potassium 483 mg, magnesium 99 mg and zinc 60 mg. The LPS content of AndoSan™ was found, using the Limulus amebocyte lysate test (COAMATIC Chromo-LAL; Chromogenix, Falmouth, MA, USA) with detection limit 0.005 EU/ml (1 EU = 0.1 ng/ml), to be a miniscule concentration of <0.5 pg/ml. The results from tests for heavy metals were conformable with strict Japanese regulations for health foods. AndoSan™ had been heat-sterilized (124°C for 1 h) by the producer. Since this mushroom extract is a commercial product, the method for its production is a business secret. Ovalbumin (OVA) (Sigma, St. Louis, MO, USA; cat.no. A7641) and Al(OH)3 were dissolved in PBS of pH 7.3, and each animal was immunized with 10 μg of OVA and 2 mg of Al(OH)3 in a total volume of 0.5 ml in the tail base.
Scheme for experimental design in murine allergy model
# Mice, strain
Treatment before and/or after OVA immunization
AndoSan™ or PBS p.o.* (200 μl)
AndoSan™/PBS p.o. and/or
AndoSan™/PBS s.c. in foot pad (20 μl)**
OVA (10 μg) + Al(OH)3 s.c. in tailbase or foot pad +
Spleen cell cultures
The spleen was removed from each sacrificed mouse and put in a tube containing Hank's Balanced Salt Solution (HBSS; Gibco BRL, Paisley, Scotland). A single cell suspension was prepared under sterile condition by placing the spleen on top of a wire-net in a Petri dish containing 2 ml HBSS. The spleen was punctured by a canula (BD Microlance™ 3 needle, Becton Dickinson AB, Sweden) and thereafter a bended glass staff was used to rub the cells from the spleen capsule through the wire net to make a single cell suspension. The cell suspensions were washed in HBSS and resuspended in RPMI (RPMI 1640 culture medium with 20 mM L-glutamine (Gibco)), containing 10% FCS, 100 U penicillin G and 0.1 mg/ml streptomycin (PAA Laboratories GmbH). The cell concentration was measured with a Coulter Counter ZI (Beckman Coulter Inc., FL, USA). The spleen cells were seeded into a 24-well culture plates (Costar Inc., NY, USA) to a final concentration of 5 × 106 cells/ml. OVA or Con A were added to a final concentration of 1 mg/ml and 6 μg/ml, respectively, except for unstimulated controls. The cells were cultured at 37°C and in 5% CO2 for 48 or 72 hours. Thereafter the plates were centrifuged at 1200 rpm for 5 minutes, and supernatants were collected and stored at -80C until analysis.
Mouse IgE anti-OVA and IgG2a anti-OVA antibodies were measured in serum, and levels of cytokines IFNγ, IL-2 (Th1 response), IL-4, IL-5 (Th2 response) and IL-10 (Treg cytokine) in cell culture supernatants by ELISAs. Whereas the former Ig ELISAs were in-house (sandwich anti-OVA IgE and simple anti-OVA IgG2a ) and the cut-off set to give negative results in serum from naïve mice, the ones for the cytokines were from R&D Systems, Minneapolis, MN, USA. The excised PLN from both injected and non-injected hind limb were weighed and compared as a parameter for local inflammation.
Sigma Stat (Systat Software, Inc., 1735 Technology Drive Suite 430 San Jose, CA) statistical and graphics package was used. When the data were normally distributed parametric assays were used, otherwise non-parametric assays. Student's t-test was used for comparing two groups. One-way ANOVA was used for single repeated measurements, and two-ways ANOVA for two experiments with repeated measurements. P values below 0.05 were considered statistically significant.
Serum anti-OVA IgE and IgG2a antibodies
Cytokines in spleen cell cultures and weight of PLN
Cytokines in supernatants of cultured spleen cells from mice treated with AndoSan™ or PBS p.o. before or after sensitization against OVA s.c..
1.13* ± 0.06
0.90* ± 0.11
1.06*, ** ± 0.13
1.07 ± 0.00
0.95 ± 0.15
0.82* ± 0.10
0.83 ± 0.08
0.78 ± 0.05
0.88* ± 0.08
0.89 ± 0.01
Our results are strengthened by the similar findings, observed in two different mouse strains after s.c. injection of OVA in the tail base, and in a third mouse strain (Balb/c) after s.c. injection of both mushroom extract and OVA in the foot pad. In the latter Th2-prone mice the so-called PLN assay was used, which was originally employed for toxicological screening of substances that would inflame the foot pad-draining PLN, but which is also convenient for examining systemic IgE response in serum to an allergen given with adjuvant . The lacking increase in PLN weight in mice injected AbM extract relative to PBS in the foot pad, agrees with the assumed anti-inflammatory anti-allergic effect of the AbM as seen from the tendency of generally lowering of Th2 cytokine levels in spleen cell cultures ex vivo.
Increased specific IgE levels are not equivalent with allergic disease, but a prerequisite for IgE-mediated allergy. Hence, our findings of decreased anti-OVA IgE levels secondary to AbM intake in animals that were otherwise sensitized to OVA, strongly indicates a protective effect of AbM against IgE-mediated allergy. We did not examine allergy signs in the mice. These would have been similar to egg allergy, as in food allergy. Possible skin rashes would have been difficult to assess in the mice, and nude mice could not have been used because they lack normal lymphocytes, which are a prerequisite for an allergic immune response. In possible follow-up studies, the allergen should be given via the natural route; e.g. p.o. if using ovalbumin, although this would be costly. Instead, a common food allergen like peanut could have been used, or if one wished to examine airways allergy in the case of aeroallergens, another cheap aeroallergen like birch pollen, although with novel ELISAs for these antigens. The finding of relatively far lower anti-OVA IgE levels in the repeated PBS controls in Figure 5, may be due to the stress invoked by such repeated intragastric procedure. In preliminary experiments, in which repeated pre-OVA treatment of mice with the mushroom extract or PBS was delivered intragastrically by the highly trained technicians to increase the dose, all mice looked sick and one animal died, presumably from stress, which is known to impair immunity.
Previously, we have used pure β-glucans from yeast and fungi together with ovalbumin s.c. in the very same PLN model and, contrary to the present observation, found increased specific anti-OVA IgE levels in serum [12, 13]. Hence, either the administration route is critical, or the particular β-glucans of the current mushroom extract does either promote a different outcome than the other β-glucans, or other stronger anti-allergic immunomodulating substances in the mushroom extract do overcome a possible general "pro-allergic" effect of β-glucans. If the latter is true, we assume that the anti-allergy effects of the AbM extract in vivo is mediated via immunomodulating substances in the extract that are smaller and more readily absorbable than β-glucans.
As to possible side effects, there are conflicting reports regarding the effect of AbM on liver function. Whereas one report suggests that use of AbM for several weeks may have induced severe hepatic dysfunction in three cancer patients , another says that AbM extract normalized liver function in patients with chronic hepatitis B virus infection . Moreover, our studies on patients with chronic hepatitis C virus infection  and on AbM intake in healthy volunteers , revealed no pathological effect whatsoever on hematological parameters including those for liver-, pancreatic- and renal function, even when volumes equivalent by body weight to that given to the mice, were taken.
The generally observed AbM-induced all-over reduction in Th2 cytokines IL-4 and IL-5 relative to Th1 cytokines IFNγ and IL-2 production ex vivo in our present cultures of spleen cells, agrees with the original Th1/Th2 dichotomy . However, this theory has been modified towards suggesting that T regulatory cells are crucial for fine-tuning both Th1 and Th2 responses by the regulatory cytokines IL-10 and TGF-β. However, our measurement of suggestive reduced levels of the Treg cytokine IL-10 in the AbM groups, is difficult to interpret. In contrast, when the extract was given in vitro to cell cultures there was an increase in proinflammatory cytokines . This apparent discrepancy must be due to the fact that whereas cells in vitro are subjected to all substances in the extract including β-glucans with large m.w., which are abundant in AbM , mainly smaller substances are taken up from the digestive tract in humans and are active in the blood in vivo. Although, β-glucans in the intestines could stimulate Peyer's patches in jejunum, we have in fact observed that the genes in leukocytes predominantly affected by AbM in vitro and in vivo were quite different [26, 28]. Whereas genes related to proinflammatory cytokines were strongly induced in vitro – presumably by β-glucan, genes involved in cell signalling and cycling and transcriptional regulation and thus foremost related to anti-tumor defence, were upregulated in vivo . Thus, the microarray analyses agree with the assumption that AbM extract especially promotes a Th1 anti-tumor and anti-infection response in the body and hence reciprocally inhibits a Th2 response. This is supported by the reported immuno-modulatory effects of AbM in mice .
β-glucans may stimulate macrophages and other cells of innate immunity after binding to cellular receptors like CD11b/18, Toll-like receptor and dectin-1 [reviewed in ]. Stimulation by AbM of peripheral blood leukocytes resulted both in an upregulation of such receptors [26, 28, 29], activation of NFKB via TLR2 stimulation , and mediation via them of increased release of proinflammatory cytokines  and Th1 cytokines IFNγ, IL-12, and IL-23α [16, 28, 31]. Although one report of reduced release of Th2 cytokine IL-4 after AbM stimulation in vitro also found reduced IL-2 and IFNγ levels , IL-12- and IFNγ-mediated NK cell activation by AbM p.o. has been documented in mice . Even though the present AbM extract should occasionally give reduced IFNγ levels, the increased expression of the IFN receptor gene after AbM extract intake in humans , may overcome a reduction in the concentration of the ligand and result in an increased Th1 response. When measuring different cytokines in serum from humans after 12 days intake of the current AndoSan™ extract mainly containing AbM, there was a significant reduction in both pro-inflammatory, Th1 and Th2 cytokines . This indicates a general anti-inflammatory effect of AndoSan™ in vivo, which agrees with its current anti-allergic effect.
For intragastric delivery of AbM extract a volume of 200 μl was chosen because this is, according to our veterinary, the maximal ventricular volume in a 5–6 weeks old mouse. In an initial experiment, we tried to give the AbM extract repeatedly on subsequent days via a gastric catheter in order to possibly inhibit the specific IgE response completely. However, this procedure was dropped because it was too stressful for the mice even in hands of our well-trained technicians. The unexpected result in the last two columns of Figure 5 may in fact reflect this concern. Also, we did not use a higher concentration of this extract than what was sold on the health food market. If translated to human intake, the equivalent of 200 μl to a 25 g mouse would be 560 ml to a 70 kg individual. In fact, a daily low intake in healthy volunteers of 60 ml AndoSan™ for 12 days gave a significant 50% reduction in levels of the allergy-promoting cytokine IL-4 in blood and left the other allergy-related cytokines IL-5, IL-7 and IL-13 at negligible levels . Addition of AbM extract to drink water for the mice in our set-up would have been more natural, but the intake of AbM is impossible to monitor as accurately as with intragastric delivery. In the current allergy model we did not test other extracts of AbM from other manufacturers that did not have a significant effect against pneumococcal infection in mice . Therefore, the question is not fully answered as to whether there is an absolute link between the anti-bacterial infection and anti-allergy effect of a substance or an extract like AndoSan™. Moreover, even though we have seen that Agaricus bM is the main TLR2 stimulating mushroom of AndoSan™ , it is likely that the former anti-bacterial and current anti-allergic effect of this mixed mushroom product may be partly due to possible synergistic effects of the other mushrooms, Hericium erinaceum and Grifola frondosa, and components thereof contained in the extract. The mouse model of allergic airways disease should be used in a follow-up study with OVA and the mushroom extract in order to confirm that also allergic symptoms like development of airways hyper responsiveness are reduced by AndoSan™ intake. Whether the AbM extract is effective against allergy in the human setting must be tested in a clinical trial, e.g. in persons with aeroallergy during the pollen season taking 60 ml a day for a few weeks.
From our results with mice we conclude that a mushroom extract, mainly containing AbM, may prevent the development of IgE-mediated allergy when given before allergen immunization. Even more interesting, the extract seemed to have a therapeutic effect when given together with or as late as 3 weeks after the allergen immunization. Three weeks in the mouse equals several months in a human, suggesting that also established allergy in patients can be reverted.
We thank Else-Carin Groeng, Åse Eikeset, Bodil Hasseltvedt and Berit Steinsby and personnel at The animal facilities, Norwegian Institute of Public Health, Oslo, for excellent technical assistance. The Norwegian Ashma and Allergy Foundation and The Foundation for Health and Rehabilitation, Norway, supported this work financially.
- Wasser SP, Weis AL: Therapeutic effects of substances occurring in higher basidiomycetes mushrooms: a modern perspective. Crit Rev Immunol. 1999, 19: 65-96.PubMedGoogle Scholar
- Huang N: Brazilian mushroom (Gee Song Rong). In Cultivation of eight rare and precious gourmet mushrooms Edited by: Huang. Chinese Agriculture University Press; 1997:95-101.
- Ohno N, Furukawa M, Miura NN, Adachi Y, Motoi M, Yadomae T: Antitumor beta glucan from the cultured fruit body of Agaricus blazei. Biol Pharm Bull. 2001, 24: 820-828. 10.1248/bpb.24.820View ArticlePubMedGoogle Scholar
- Firenzuoli F, Gori L, Lombardo G: The medicinal mushroom Agaricus blazei Murill: Review of literature and pharmaco-toxicological problems. Evid Based Complement Alternat Med. 2008, 5: 3-15. 10.1093/ecam/nem007PubMed CentralView ArticlePubMedGoogle Scholar
- Itoh H, Ito H, Amano H, Noda H: Inhibitory action of a (1-->6)-beta-D-glucan-protein complex (F III-2-b) isolated from Agaricus blazei Murill ("himematsutake") on Meth A fibrosarcoma-bearing mice and its antitumor mechanism. Jpn J Pharmacol. 1994, 66: 265-271. 10.1254/jjp.66.265View ArticlePubMedGoogle Scholar
- Bernardshaw S, Johnson E, Hetland G: An extract of the mushroom Agaricus blazei Murill administered orally protects against systemic Streptococcus pneumoniae infection in mice. Scand J Immunol. 2005, 62: 393-398. 10.1111/j.1365-3083.2005.01667.xView ArticlePubMedGoogle Scholar
- Bernardshaw S, Hetland G, Grinde B, Johnson E: An extract of the mushroom Agaricus blazei Murill protects against lethal septicemia in a mouse model for fecal peritonitis. Shock. 2006, 25: 420-425. 10.1097/01.shk.0000209526.58614.92View ArticlePubMedGoogle Scholar
- Romagnani S: The Th1/Th2 paradigm. Immunol Today. 1997, 18: 263-266. 10.1016/S0167-5699(97)80019-9View ArticlePubMedGoogle Scholar
- Seljelid R: A water-soluble aminated beta-1-3D-glucan derivative causes regression of solid tumores in mice. Biosci Rep. 1986, 6: 845-851. 10.1007/BF01117108View ArticlePubMedGoogle Scholar
- Reynolds JA, Kastello MD, Harrington DG, Crabbs CL, Peters CJ, Jemski JV, Scott GH, Di Luzio NR: Glucan-induced enhancement of host resistance to selected infectious diseases. Infect Immun. 1980, 30: 51-57.PubMed CentralPubMedGoogle Scholar
- Hetland G, Ohno N, Aaberge IS, Løvik M: Protective effect of β-glucan against systemic Streptococcus pneumoniae infection in mice. FEMS Immunology and Medical Microbiology. 2000, 27: 111-116.PubMedGoogle Scholar
- Ormstad H, Groeng EC, Løvik M, Hetland G: The fungal cell wall component β-1, 3-glucan has an adjuvant effect on the allergic response to ovalbumin in mice. J Toxicol Environ Health Part A. 2000, 61: 55-67. 10.1080/00984100050116780View ArticlePubMedGoogle Scholar
- Instanes C, Ormstad H, Rydjord B, Wiker HG, Hetland G: Mould extracts increase the allergic response to ovalbumin in mice. Clin Exp Allergy. 2004, 34: 1634-1641. 10.1111/j.1365-2222.2004.02076.xView ArticlePubMedGoogle Scholar
- Wan GH, Li CS, Guo SP, Rylander R, Lin RH: An airborne mold-derived product, beta-1, 3-D-glucan potentiates airway allergic responses. Eur J Immunol. 1999, 29: 2491-2497. 10.1002/(SICI)1521-4141(199908)29:08<2491::AID-IMMU2491>3.0.CO;2-RView ArticlePubMedGoogle Scholar
- Bernardshaw S, Hetland G, Ellertsen LK, Aaland Tryggestad AM, Johnson E: An extract of the medicinal mushroom Agaricus blazei Murill differentially stimulates production of pro-inflammatory cytokines in human monocytes and human vein endothelial cells in vitro. Inflammation. 2005, 29: 147-153. 10.1007/s10753-006-9010-2View ArticlePubMedGoogle Scholar
- Yuminamochi E, Koike T, Takeda K, Horiuchi I, Okumura K: Interleukin-12- and interferon-gamma-mediated natural killer cell activation by Agaricus blazei Murill. Immunology. 2007, 121: 197-206. 10.1111/j.1365-2567.2006.02558.xPubMed CentralView ArticlePubMedGoogle Scholar
- Hetland G, Johnson E, Lyberg T, Bernardshaw S, Tryggestad AMA, Grinde B: Effects of the medicinal mushroom Agaricus blazei Murill on immunity, infection and cancer. Scand J Immunol. 2008, 68: 363-370. 10.1111/j.1365-3083.2008.02156.xView ArticlePubMedGoogle Scholar
- Suehiro M, Kishimoto S: Cheilitis due to Agaricus blazei Murill mushroom extract. Contact Derm. 2007, 56: 293-294. 10.1111/j.1600-0536.2006.01013.xView ArticlePubMedGoogle Scholar
- Chan Y, Chang T, Chan CH, Yeh YC, Chen CW, Shieh B, Li C: Immunomodulatory effect of Agaricus blazei Murill in Balb/cByJ mice. J Microbiol Immunol Infect. 2007, 40: 201-208.PubMedGoogle Scholar
- Tanaka H, Tsunematsu K, Makamura N, Suzuki K, Tanaka N, Takeya I, Saikai T, Abe S: Successful treatment of hyper-sensitivity pneumonitis caused by Grifola frondosa (Maitake) mushroom using a HFA-BDP extra-fine aerosol. Intern Med. 2004, 43: 737-740. 10.2169/internalmedicine.43.737View ArticlePubMedGoogle Scholar
- Maes MF, van Baar HM, van Ginkel CJ: Occupational allergic contact dermatitis from the mushroom White Pom Pom (Hericeum erinaceum). Contact Derm. 1999, 40: 289-290. 10.1111/j.1600-0536.1999.tb06073.xView ArticlePubMedGoogle Scholar
- Hetland G: Applicant/Inventor: Use of the mushroom Agaricus blazei Murill for the production of medicaments suitable for treating infections and allergies. WO 2005/065063 (Priority; Jan 2004)
- Løvik M, Hogseth AK, Gaarder PI, Hagemann R, Eide I: Diesel exhaust particles and carbon black have adjuvant activity on the local lymph node response and systemic IgE production to ovalbumin. Toxicology. 1997, 121: 165-178. 10.1016/S0300-483X(97)00075-9View ArticlePubMedGoogle Scholar
- Mukai H, Watanabe T, Ando M, Katsumata N: An alternative medicine, Agaricus blazei, may have induced severe hepatic dysfunction in cancer patients. Jpn J Clin Oncol. 2006, 36: 808-810. 10.1093/jjco/hyl108View ArticlePubMedGoogle Scholar
- Hsu CH, Hwanq KC, Chiang YH, Chou P: The mushroom Agaricus blazei Murill extract normalizes liver function in patients with chronic hepatitis B. J Altern Complement Med. 2008, 14: 299-301. 10.1089/acm.2006.6344View ArticlePubMedGoogle Scholar
- Grinde B, Hetland G, Johnson E: Effects on gene expression and viral load of a medicinal extract from Agaricus blazei in patients with chronic hepatitis C infection. Int Immunopharmacol. 2006, 6: 1311-1314. 10.1016/j.intimp.2006.04.005View ArticlePubMedGoogle Scholar
- Johnson E, Førland DT, Sætre L, Bernardshaw SV, Lyberg T, Hetland G: Effect of an extract based on the medicinal mushroom Agaricus blazei Murill on release of cytokines, chemokines and leukocyte growth factors in human blood ex vivo and in vivo. Scand J Immunol. 2009, 69: 242-250. 10.1111/j.1365-3083.2008.02218.xView ArticlePubMedGoogle Scholar
- Ellertsen LK, Hetland G, Johnson E, Grinde B: Effect of a medicinal extract from Agaricus blazei Murill on gene expression in a human monocyte cell line as examined by microarrays and immuno assays. Int Immunopharmacol. 2006, 6: 133-143. 10.1016/j.intimp.2005.07.007View ArticlePubMedGoogle Scholar
- Bernardshaw S, Lyberg T, Hetland G, Johnson E: Effect of an extract of the medicinal mushroom Agaricus blazei Murill on expression of adhesion molecules and production of reactive oxygen species in monocytes and granulocytes in human whole blood ex vivo. APMIS. 2007, 1157: 19-25.Google Scholar
- Tryggestad AMA, Espevik T, Førland DT, Ryan L, Hetland G: The medicinal mushroom Agaricus blazei Murill activates NF-KB via TLR2. 13th Int. Congress of Immunology, Rio de Janerio, 2007:P2.23 INI-02 Signalling pathways of innate immune receptors; abstract P1193.Google Scholar
- Kasai H, He LM, Kawamura M, Yang PT, Deng XW, Munkanta M, Yamashita A, Terunuma H, Hirama M, Horiuchi I, Natori T, Koga T, Amano Y, Yamaguchi N, Ito M: IL-12 Production Induced by Agaricus blazei Fraction H (ABH) Involves Toll-like Receptor (TLR). Evid Based Complement Alternat Med. 2004, 1: 259-267. 10.1093/ecam/neh043PubMed CentralView ArticlePubMedGoogle Scholar
- Kuo YC, Huang YL, Chen CC, Lin YS, Chuang KA, Tsai WJ: Cell cycle progression and cytokine gene expression of human peripheral blood mononuclear cells modulated by Agaricus blazei. J Lab Clin Med. 2002, 140: 176-187. 10.1067/mlc.2002.126717View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.